A Multiview 1000 was used to probe the elasticity  of fixed macrophage cells with force spectroscopy.  Force spectroscopy was conducted on various cells with measurements performed around the nucleus.  A 3D image of a sample cell is shown below, with the force curves conducted at designated points on top of the cell.  Sneddon’s model was used to characterize the individual force curves to measure the elastic indentation of the cells; the Young’s modulus can then be calculated  by fitting the Hertz model to the data.  The authors find that the modulus of the macrophage cells increased significantly with adhesion to the ECM, showing that cellular adhesion plays a significant role in cytoskeleton and membrane softness.

Published:  Souza et al., European Biophysics Journal, 2014 DOI 10.1007/s00249-014-0988-3

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