Yeast Cells
Zooming from Far-field to Near-field

Far-field optical images of yeast cells in a liquid cell 

X5 optical image		X10 optical image
X20 optical image		X50 image with the fiber probe in place illuminating the sample

   
Nanometric scale images obtained without moving anything

7x7micron AFM Topgraphy		NSOM GFP Fluorescence		NSOM Transmission

    
These three images show the same region of GFP labelled yeast cell in a physiological medium. The images were obtained simultaneously with the MultiView 1000 system integrated with a Nanonics dual microscope.

Only Nanonics' systems can zoom in on living samples from the far-field to NSOM producing images of such high quality.

The simultaneous imaging of a sample in both far- and near-fields, from above and below the sample can be achieved only by virtue of the Nanonics' free optical axis.

NSOM imaging of biological samples such as these is possible in the Nanonics liquid cell using optical fiber probes intermittant contact mode. Other NSOM techniques using hollow probes are limited in their ability to image in the intermittant contact mode.