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Thursday, 10 April 2014 09:23

Energy Transfer in Phycocyanin Nanowires

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Observing energy transfer and wave guiding in phycocyanin nanowires using a MV4000 Two Probe system

Near-field Luminescence Mapping 

Energy transfer via phycocyanin nanowires has been shown by measuring near-field luminescence with a MV4000 two probe system. One NSOM probe has been used for localized excitation (532 nm light, 150 nm diameter aperture) and a second NSOM tip (150nm diameter aperture) has been used simultaneously to map AFM and Luminescence along the nanowire.
The MV4000 allows for accurate placement of the NSOM illumination probe on top of the nanowire and also allows for simultaneous tip-scanning in close proximity to the illumination tip.


Energy transfer measurement with a two probe MV4000 system.  (a) Optical microscope image of Phycocyanin nanowire. This sample was scanned by a two probe NSOM system for measuring energy transfer. Excitation was done by a 532 nm wavelength laser through a 150 nm diameter NSOM tip and detection was done with a multimode fiber with a 250 nm diameter tip. Excitation was done at the green circle in a)and collecting was done within the black frame. When comparing the excitation distribution (b) and the luminescence distribution (c) clear broadening is obtained. (d) Cross-section of these two graphs at y = 9.7 um shows that the luminescence (red) Full-Width-Half-Maximum (FWHM) is wider than the excitation (green) FWHM in more than 2 um

 Published: Phys. Chem. Chem. Phys., 2014. DOI: 10.1039/c4cp00345d  









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