AFM/NSOM Imaging of Fibroblast Cells in Liquid Cell
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| (A) AFM topographic imaging with simultaneous NSOM imaging (B) with an AFM/NSOM cantilevered fiber probe at transmission mode (50x objective of an inverted optical microscope) in liquid cell |
AFM of similar fibroblast cells with an AFM nominally designed for BioAFM
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Topography (Left) and NSOM Images (Right). Scan Range 50x50 Microns
Unparalleled Scanned Probe Cellular Imaging
Fully Integrated with Optical Microscopy
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CCD image of the sample with SPM/NSOM probe at the cell surface. The point of SPM imaging can be seen clearly in the optical microscope.
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NSOM image of 50x50 µm
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NSOM image of 50x50 µm
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NSOM image of 8x8 µm
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These images illustrate the ability of Nanonics' MultiView systems to overcome the difficulties usually associated with viewing live, soft tissue liquid samples. Nanonics' SPM systems allow facile viewing of liquid samples via a versatile and user-friendly liquid cell attachment. The open architecture of Nanonics' SPM heads allows the user to view samples from above and below. These systems are easily integrated into Nanonics' dual microscope which combines both upright and inverted formats. This is particularly important for better resolution of low-contrast biological samples.
The free optical axes also allows fluorescence and Raman imaging to be achieved with ease.
With the same system AFM was employed for topographical mapping. The AFM was completed in intermittent contact mode, as regular contact mode would have scratched the soft tissue. Nanonics' systems are able to operate in all three SPM modes.
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