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Super User

Wednesday, 20 February 2013 09:17

AFM Raman of Name Card

Simultaneously Obtained Topography and Raman Images of Name Card

     

Topography image

Raman image at 1547 cm-1

Raman image at 954 cm-1

Wednesday, 20 February 2013 09:10

AFM and NSOM of A Multi Mode Fibre

 

 

 

A) AFM topographic image of a cleaved multimode optical fiber obtained with AFM/NSOM probe. 
B) A correlating NSOM image in collection mode obtained simultaneously with (A). 
C) A 3D collage AFM/NSOM of the output optical distribution with exact correspondence to the surface’s topography
.

 

  • Near-field optical distribution of a multimode optical fiber launched with 532nm laser.

  • The Nanonics' 3D FlatScanTM stage allows for vertical mounting of the optical 

    fiber with a flexible geometry for optical microscopy integrations.

  • The sample is kept stationary along the scan to prevent any disturbance of the light propagation through the fiber. Nanonics systems with Tip-Scanning capabilities are ideal in such applications for true profiling of the optical output. 
     
  • The AFM/NSOM fully correlated imaging is based on tuning fork feedback in normal mode.

 

 

Nanonics Nano3D Distortion Free, Near-field/Far-field  Beam

Profiler

 

This novel product provides high precision, 50nm optical resolution with simultaneous topography and without deconvolution. 

 

  • Unprecedented error-free profiling of divergent sources with no detector saturation or beam attenuation. 

 

  • No non-uniformities or astigmatisms in profiling active or passive sources such as VCSELs, AWGs, Ultrasmall Mode Field Diameter Lensed Fibers etc.
  • Seamless complex beam structures 3D profiles from the near-field to the far-field with overlapping fields of view.

 

 

 

Ideal systems for this application:

 

 

 

 

  An optical fiber vertically mounted on MultiView 2000TM SPM head integrated with an upright optical microscope 
Wednesday, 20 February 2013 09:00

TFT in Liquid Crystal Image

Thin Film Transistor in Liquid Crystal Display
  

     
50 x 50 micron AFM Topgraphy   Simultaneously produced NSOM image
 
 
  
 
   
   

These images was produced using the MultiView 1000™ Microscope.

For more details, see the application note on TFT Displays Click here to download (489kB)
 

Wednesday, 20 February 2013 08:47

AFM, NSOM and Capicatance SRAM Image

SRAM
AFM, NSOM and Capacitance Imaging

The first simultaneous NSOM/Capacitance ever to be produced.
  

 
10x10 micron AFM Topgraphy    NSOM Image of the same region
     

Only Nanonics Double Wire Electrodes can produce simultaneous NSOM/Capacitance measurements

 
These images were obtained by the MultiView 1000™
 
 
Simultaneous Capacitance Image of the same region
Wednesday, 20 February 2013 08:16

High Resolution NSOM Imaging in Reflection mode

   

 

 

 

 

 

A) Topographic AFM image of a Photonics Band Gap (PBG) structure, imaged with a 150nm AFM/NSOM cantilevered probe. 

B) Online correlated NSOM image of the PBG structure at (A). Imaged with the same probe in reflection mode at a 532nm laser wavelength.

C) NSOM line profile shows a lateral resolution of 100nm in reflection mode.
  • Nanonics unique, spatially friendly glass based probes allow for free optical axis from the top for NSOM imaging in reflection mode. Unlike other NSOM techniques, the Nanonics approach exclusively integrates  all MultiView SPM series with all types of optical microscopy including upright reflection microscopes, without obscuring the  optical axis from above. 
  • AFM and NSOM images are simultaneity acquired with the same probe to obtain fully correlated topographic and NSOM data without any need to change the probe.
  • For both feedback mechanisms of beam bounce and tuning fork, AFM/NSOM imaging is performed in normal force mode for the ultimate in sensitivity.
  • Nanonics' unique reflection NSOM mode is the standard for imaging opaque samples of photonic Si structures and microelectronics. All are easily characterized in reflection mode of an upright microscope without the need for complicated geometries.   
  • As demonstrated above, Nanonics provides the ultimate NSOM resolution in reflection mode of less than 100nm.

 

 

Ideal systems for this application: 

 

1. MultiView 1000 
2. MultiView 2000

3. MultiView 4000

4. CryoView 2000

 

 

Tuesday, 19 February 2013 12:13

PEO Spherulite Imaging

 
 
     
15 x 15 micron AFM image showing nucleus and lamellar
crystallites.
  Transmission NSOM image
Polarization shows the radial
dependence of the birefringence
     
 
Simultaneously obtained Confocal image of the same region. The highlighted area is the 15x15micron region imaged above.
 
Nanonics systems can perform simultaneous NSOM and Confocal microscopy the highest quality available today.

The Nanonics MultiView 1000™ is designed around  the 3D Flatscan™ which has a completely free optical axis from above and below the sample. Because Nanonics uses cantilevered optical fiber probes the microscope objective is not blocked by the probe.

This unique technology allows the MultiView 1000™ to be integrated into the Nanonics Dual Microscope which can simultaneously produce conventional confocal microscopy images along side transmission NSOM images

Tuesday, 19 February 2013 12:05

High Resolution NSOM Imaging of Au Grid

 
   
 A) AFM image of an Au grid glass surface obtained with an AFM/NSOM probe.                                     B) NSOM image obtained in transmission mode fully correlated with A.

 

   
   

Upper Graph: Shows the height line profile of image A. Notice the high resolution of the AFM image despite its being obtained with an NSOM probe.

Bottom Graph: Shows the NSOM line profile of image B. The cursors show a high lateral resolution of 50nm.

Tuesday, 19 February 2013 11:40

Murine Stem Cell Imageing

 

 

 

 

AFM and NSOM Flourescence of Murine Stem Cells

 

 

typical Z height approx. 18 microns

Flourescence 

AFM                    

 

 

 

 

 

 

 

 

AFM and NSOM Transmission of Murine Stem Cells

  typical Z height approx. 18 microns

Transmission

                                             AFM            

 

 
  The only difference between these images and the images above is the wavelength of the laser.
Tuesday, 19 February 2013 10:50

Human Fibroblast Imaging

 

 

 

 

AFM-NSOM Measurements of Healthy Human

Dermal Fibroblasts in Liquid

A demonstration of Nanonics' unique abilities in both biological and soft sample imaging.

The imaging of soft tissues, such as the live fibroblast cells shown below, leads the way to many new biological applications. The human dermal cells were imaged live in a PBS buffer (pH-7.3).  Below we see a chain of NSOM images obtained in transmission mode with a Nanonics patented cantilevered tip with  an aperture diameter of 100nm.

 

     

50 x 50 microns

30 x 30 microns

8 x 8 microns


These images illustrate  the abilities of Nanonics' MultiView systems to overcome the difficulties usually associated with viewing live, soft tissue liquid samples. These SPM systems permit easy viewing of liquid samples via a versatile and  user-friendly liquid cell attachment. The open architecture of Nanonics' SPM heads allows one to view samples from above and below. These systems are easily integrated into our dual microscope which combines both upright and inverted formats. This is particularly important for better resolution of low-contrast biological samples.

The free optical axes also allows fluorescence or Raman imaging to be accomplished with ease.

 Using the same system AFM was used for topographical mapping. The AFM was carried out in intermittent contact mode, as regular contact mode would have scratched the soft tissue. Nanonics systems are able to operate in all three SPM modes.

 

 

CCD sample image with NSOM probe approaching  the surface




 

Below we see the AFM images (left) and the corresponing (right) NSOM images.

 

   
   
                                              AFM images (left) NSOM images (right) 
                                                          done at 50 x 50 microns

 

 

 

Below is shown a line scan of simultaneously obtained topography and NSOM images. The AFM image is at the top and the NSOM image is at the bottom.
  

 

   
   

 

 

Human dermal fibroblasts are derived from the dermis of normal human skin and have been used for in vitro analyses of fibroblast growth, migration and collagen metabolism in wound healing. Fibroblasts are responsible for the homeostasis of connective tissues and their activity is modulated by several cells and soluble factors. SPM techniques using our MultiView systems were used as part of a study to investigate the factors that regulate the synthesis of the extracellular matrix molecules and the functional organization of these molecules in the extracellular space.

 

 

 

 

 

 

 

AFM/EFM imaging of graphene transistor. Height AFM image (upper left) and correlated Electrostatic Force Microscopy imaging performed with Nanonics Pt nanowire electrical probe (Upper right Image). The graphene is connected to two separate gold electrodes (upper right and lower left corners) which have been biased with 1.5v Lower Left Image is 3D presentation of the AFM image . Lower Right Image is AFM/EFM 3D collage presentation

   
AFM 2D Closeup EFM 2D closeup
   
AFM 2D Closeup AFM/EFM Collage

 

 

Performed with Nanonics unique ultrastable Nano-wire glass insulated electrical probe.

 

  • Ultrastable solid wire electrical probes q Ultra-Sensitive Tuning Fork Normal Force feedback
  • Geometrical friendly for online multiprobe all under active AFM feedback.
  • Low contact resistance and full insulation with glass upto the probe tip for high electro-potential resolution
  • Glass coating insulation can be overcoated with metal to emulate coax geometries for ultrahigh sensitivity electrical imaging
  • High cantilever design that minimizes cantilever electrical interference

 

Find the Scanning Probe Microscope that's right for you.

 

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