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Chromosome Nanomanipulation

 

 

 

Nanonics' systems can use any type of probe, but our unique glass probes are perfect for chromosome nanomanipulation and imaging. Glass probes do not only allow for the imaging of soft structures such as this human chromosome but are also ideal, because of their slender and extended shape, for the precise dissection of such structures.

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AFM of Insulin

 

5x5 microns

 

2x2 microns

The measurements for these images were obtained using the MV400 head and a cantilevered AFM probe with a tip diameter of 10nm. Each image is 256 pixels.

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AFM Imaging Double Strand Lambda DNA

     

 

 

A:  2x2 µm AFM topographic image of Double Strand Lambda DNA immobilized on Mica surface with Mg ions
B:  AFM phase image of A
C:  464.8x464.8 µm AFM topographic image  of the same sample

 

 

   D:  Topographic line profile (shown in C) showing a 12nm lateral resolution
 
 

E:  3D topographic presentation of C

WSxM software has been used for image processing of the pictures above: I. Horcas et al. Rev. Sci. Instrum. 78, 013705 (2007).

 

 

Ideal systems for this application:

1. MultiView 1000 
2. MultiView 2000 
3. MultiView 4000 

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collagen imaging

 
 
 
  2D and 3D AFM images of Collagen Fibrilis imaged with the optically friendly Nanonics' OptoprobeTM. The images shown clearly repeat the structure of 65nm.

 

 

 

 

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Molecular Pentacene

 

   AFM (2.5x2.5µm) soft imaging of Molecular Pentacene

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Yeast Cells Fluorescence

Yeast Cells 
Zooming from Far-field to Near-field


Far-field optical images of yeast cells in a liquid cell 

     
X5 optical image   X10 optical image
     
     
X20 optical image   X50 image with the fiber probe in place illuminating the sample  
   
Nanometric scale images obtained without moving anything 
         
7x7micron AFM Topgraphy   NSOM GFP Fluorescence    NSOM Transmission

    
These three images show the same region of GFP labelled yeast cell in a physiological medium. The images were obtained simultaneously with the MultiView 1000 system integrated with a Nanonics dual microscope.

 

Only Nanonics' systems can zoom in on living samples from the far-field to NSOM producing images of such high quality.

The simultaneous imaging of a sample in both far- and near-fields, from above and below the sample can be achieved only by virtue of the Nanonics' free optical axis.

NSOM imaging of biological samples such as these is possible in the Nanonics liquid cell using optical fiber probes intermittant contact mode. Other NSOM techniques using hollow probes are limited in their ability to image in the intermittant contact mode.

 
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BSA Protein Nanoarray Writing

 
 
4X4 micron Image of Protein dots printed with a Nanofountain Pen

Protein printing is made possible by the Nanonics Chemical Delivery System and NanopipetteOnly Nanonics can deliver chemicals or gas onto the sample on line, with no need to remove the tip from the sample.


Nanofountain Pen reviewed by Nature Materials

In the August 2003 edition of Nature Materials. The Nanofountain Pen was reviewed in the Material Update section.

 

 
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BSA Protien Writing

 

 

 

 

 

 
60 x 80 micron optical image of the same region.
 
 
Video of protein printing in real time
     

  
Only Nanonics can deliver chemicals or gas onto the sample on line, with no need to remove the tip from the sample.

Protein printing is made possible by the Nanonics Chemical Delivery System and Nanopipette

 

Using any of the Nanonics MultiView systems, chemicals, in liquid or gas form, can be fed into the pipette via a silicon tube. Apart from Protein Printing, applications of this setup include metallic nano-etching and nanolithography: an etchant can be introduced into the sample and scanned across it with nanometer precision using our 3D FlatScanning™ technology. The nanopipette is engaged by the sample using standard contact-mode atomic force microscopy, and our integrated system makes it possible to view the etching simultaneously through any optical microscope.


 

    Nanofountain Pen reviewed by Nature Materials

In the August 2003 edition of Nature Materials. The Nanofountain Pen was reviewed in the Material Update section.

Click to download application note on the uses of the nanofountain pen (331kB)

 

 

 
 

Neuronal Response to Caffeine Using a Chemical Probe in SPM 

 

 
Functional Calcium Imaging - An Optical Alternate to Patch Clamping

 

  • In this series of images a Nanopipette probe containing OrangeGreen is held in place with AFM control over a neuronal cell membrane of a cell which is filled with Fluo3.
  • The probe tip and the cell are excited in epi-illumination.
  • Each frame in the image is one confocal scan with 250 millisecond intervals between frames.  
  • A puff of caffeine is injected just before the fourth image (top row).
  • The cell and tip respond to the caffeine peaking in the center of the second row. Some of the pixels are red indicating that calcium has flowed out of the membrane and can be found at the tip which is in close proximity to the cell membrane.
  • Only Nanonics produces such probes for chemical imaging.
     
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Monkey's Kidney

Monkey Kidney Cells 
Fluorescence NSOM Images
  
 

     
35x35 micron AFM image    35x35 micron Transmission FluorescenceNSOM image

These images were produced using in the Intermittant Contact mode in a liquid environment using the Nanonics Liquid Cell with the MultiView 1000 system.

 

These topographic and fluorescence images were obtained simultaneously with cantilevered NSOM fiber optic probes. Labelled with rabbit anti-fibronectin primary antibody which was followed by a goat anti-rabbit Alexflour 488 secondary antibody. The Alexaflour probe is excited at about 488 and emits approximately 520nm. Thus, all fibronectin should be fluorescent. Fibronectin is part of the extracellular matrix which attaches the cell to the glass substrate.

The cell is visible in the AFM topography image but the fluorescent image shows mainly fibers. The fibers are primarily under the cell and therefore are difficult to image with AFM unless the cell is thin enough and these cells are thick since they were imaged at a more advanced stage in their growth. However, the fibers are fluorescent so they show up in the fluorescence NSOM optical image. 

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