Fluorescence NSOM

Fluorescence NSOM 

Traditional fluorescence instruments including confocal microscopy do not have enough optical resolution for detection of nanoscale fluorescence objects.

NSOM fluorescence is a sensitive nano-optical technique which makes use of the fluorescence for near-field imaging and is especially suited for biological applications. Fluorescence is excited by a laser transmitted through the nanometric aperture of an NSOM probe and the emitted fluorescence is collected in far-field. Appropriate optical filters are used for removal of all primary laser light and detection of the emitted fluorescence. Optical resolution of NSOM fluorescence imaging is a function of the NSOM probe aperture and can be less than 50 nm. Topographic and fluorescence images are acquired simultaneously. Transmission or reflection fluorescence NSOM modes are used depending on the substrate transparency.

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