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Thursday, 28 February 2013 11:45

Chondrocytes AFM Imaging in Liquid Cell

 
     

AFM imaging of Chondrocytes cells in liquid media. Middle and right images are close-up scans.  

 

The Nanonics HydraTM AFM system allows for unique Tuning Fork AFM feedback imaging in liquid environments (right). The setup provides a clear optical axis even from the top, suitable for water immersion objectives. The red marked circle in the left-hand picture shows the very end of the AFM tip during the above AFM scans.

 

Thursday, 28 February 2013 11:39

Neuroblastoma AFM Imaging

AFM imaging of Neuroblastoma cells in liquid 

AFM close-up imaging of Neuroblastoma cells in liquid  

3D AFM imaging of Neuroblastoma cells in liquid  3D AFM close-up imaging of Neuroblastoma cells in liquid

 

 

 

 

 

Thursday, 28 February 2013 11:37

Chromosome Nanomanipulation

 

 

 

Nanonics' systems can use any type of probe, but our unique glass probes are perfect for chromosome nanomanipulation and imaging. Glass probes do not only allow for the imaging of soft structures such as this human chromosome but are also ideal, because of their slender and extended shape, for the precise dissection of such structures.

Thursday, 28 February 2013 11:35

AFM of Insulin

 

5x5 microns

 

2x2 microns

The measurements for these images were obtained using the MV400 head and a cantilevered AFM probe with a tip diameter of 10nm. Each image is 256 pixels.

Thursday, 28 February 2013 11:32

AFM Imaging Double Strand Lambda DNA

     

 

 

A:  2x2 µm AFM topographic image of Double Strand Lambda DNA immobilized on Mica surface with Mg ions
B:  AFM phase image of A
C:  464.8x464.8 µm AFM topographic image  of the same sample

 

 

   D:  Topographic line profile (shown in C) showing a 12nm lateral resolution
 
 

E:  3D topographic presentation of C

WSxM software has been used for image processing of the pictures above: I. Horcas et al. Rev. Sci. Instrum. 78, 013705 (2007).

 

 

Ideal systems for this application:

1. MultiView 1000 
2. MultiView 2000 
3. MultiView 4000 

Thursday, 28 February 2013 11:29

collagen imaging

 
 
 
  2D and 3D AFM images of Collagen Fibrilis imaged with the optically friendly Nanonics' OptoprobeTM. The images shown clearly repeat the structure of 65nm.

 

 

 

 

Thursday, 28 February 2013 11:27

Molecular Pentacene

 

   AFM (2.5x2.5µm) soft imaging of Molecular Pentacene

Thursday, 28 February 2013 11:22

Yeast Cells Fluorescence

Yeast Cells 
Zooming from Far-field to Near-field


Far-field optical images of yeast cells in a liquid cell 

     
X5 optical image   X10 optical image
     
     
X20 optical image   X50 image with the fiber probe in place illuminating the sample  
   
Nanometric scale images obtained without moving anything 
         
7x7micron AFM Topgraphy   NSOM GFP Fluorescence    NSOM Transmission

    
These three images show the same region of GFP labelled yeast cell in a physiological medium. The images were obtained simultaneously with the MultiView 1000 system integrated with a Nanonics dual microscope.

 

Only Nanonics' systems can zoom in on living samples from the far-field to NSOM producing images of such high quality.

The simultaneous imaging of a sample in both far- and near-fields, from above and below the sample can be achieved only by virtue of the Nanonics' free optical axis.

NSOM imaging of biological samples such as these is possible in the Nanonics liquid cell using optical fiber probes intermittant contact mode. Other NSOM techniques using hollow probes are limited in their ability to image in the intermittant contact mode.

 
Thursday, 28 February 2013 11:19

BSA Protein Nanoarray Writing

 
 
4X4 micron Image of Protein dots printed with a Nanofountain Pen

Protein printing is made possible by the Nanonics Chemical Delivery System and NanopipetteOnly Nanonics can deliver chemicals or gas onto the sample on line, with no need to remove the tip from the sample.


Nanofountain Pen reviewed by Nature Materials

In the August 2003 edition of Nature Materials. The Nanofountain Pen was reviewed in the Material Update section.

 

 
Thursday, 28 February 2013 11:12

BSA Protien Writing

 

 

 

 

 

 
60 x 80 micron optical image of the same region.
 
 
Video of protein printing in real time
     

  
Only Nanonics can deliver chemicals or gas onto the sample on line, with no need to remove the tip from the sample.

Protein printing is made possible by the Nanonics Chemical Delivery System and Nanopipette

 

Using any of the Nanonics MultiView systems, chemicals, in liquid or gas form, can be fed into the pipette via a silicon tube. Apart from Protein Printing, applications of this setup include metallic nano-etching and nanolithography: an etchant can be introduced into the sample and scanned across it with nanometer precision using our 3D FlatScanning™ technology. The nanopipette is engaged by the sample using standard contact-mode atomic force microscopy, and our integrated system makes it possible to view the etching simultaneously through any optical microscope.


 

    Nanofountain Pen reviewed by Nature Materials

In the August 2003 edition of Nature Materials. The Nanofountain Pen was reviewed in the Material Update section.

Click to download application note on the uses of the nanofountain pen (331kB)

 

 

 
 
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