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Nanonics Press Release: August 2013

Nanonics is  proud to announce that our triple beam integrated AFM/SEM/FIB system, the Nanonics 3TB4000, was judged one of the ten best microscopy innovations in 2013 and is the recipient of the prestigious

2013 Microscopy Today Innovation Award. The 3TB4000 provides the ultimate 3D nanoscale characterization capability through a revolutionary innovation of open architecture that provides open access to the SEM/FIB beams without any obstruction or interference to the injectors, detectors, or beam lines.

With the 3TB4000, the SEM,FIB, and AFM can now be used to provide complimentary information in order to provide a complete characterization of material by taking advantage of the functional and high resolution 3D capabilities of AFM, the large field of view and rapid scanning of the SEM, and the fabrication / material removal capability of the FIB. Applications demonstrating this powerful new capability have been shown in diverse areas including side wall imaging in semiconductors, locating and measuring optical properties of individual metal oxide nanowires or mechanical properties of surface features, and assessing AFM probes in situ while imaging.


Nanonics founders Aaron and Chaya Lewis accept the  2013 Microscopy Today
Innovation Award at the 2013
 Microscopy and Microanalysis meeting in Indianapolis

       The award winning Nanonics3TB4000, an integrated
AFM/SEM/FIB system

Sunday, 11 August 2013 08:05


    East Coast   West Coast
Contact:   Scott  Barton   Steven Barnett
Phone:   +1 413 587 4000   +1 916 897 2441
Friday, 28 June 2013 09:00

Nanonics Featured on Cover of Analyst

Origin of enhanced signal from TERS measurements on functionalized nanoparticles.

Recent work by Nanonics Users Z.D. Schultz and H. Wang (Analyst, 2013, 138, 3150-3157) on understanding the origin of TERS signals from functionalized nanoparticles was featured on the front cover of Analyst and identified as a “hot article” by its editors.  In this work , the authors used a Nanonics MV4000 customized with a homebuilt Raman microscope to measure the TERS signal of gold nanoparticles functionalized with proteins and compare that with SERS measurements of clustered gold nanoparticles to understand the source of enhancement of the TERS signal and its relation to location of the enhanced molecule with respect to the gap.  The ability to understand the source of the enhancement of the TERS signal provides flexibility in preparing complicated biological samples such as receptors for TERS imaging beyond the traditional method of affixing the target to a metallic surface, which cannot be applied to many materials (e.g. biological receptors).


Surface enhanced Raman spectroscopy (SERS) is a surface sensitive technique where Raman scattering is enhanced by molecules adsorbed on a rough or nanostructured metal surface and is used for ultrasensitive detection resulting in single molecule sensitivity in a variety of fields including materials and biology.  TERS, or tip enhance Raman spectroscopy, also results in enhancement of the Raman signal and employs scanning a very sharp metallic or metalized tip to create a “nano-antenna” resulting in high resolution and enhanced Raman signal, and has been used to investigate carbon nanotubes, semiconductors, and biologically relevant molecules.  In TERS, substantial enhancement has been observed from gap-modes that arise from image dipoles as the tip approaches a metal surface. However, a limitation of gap-mode TERS is the requirement of a metallic or conducting substrate.  To address this limitation, nanoparticles on dielectric substrates interacting with the TERS tip have been shown to generate enhancement comparable to gap-modes and thus broadens the applicability of TERS with a more flexible sample preparation.

Goal and findings

The goal of this study is elucidate the location of the enhanced molecule within the gap that leads to the TERS signal, and to understand whether nanoparticles bound to proteins can provide additional TERS enhancement without the analyte of interest residing directly in the gap junction.  The authors focus on the biotin-streptavidin system, a popular ligand-receptor binding system.

This work suggests that TERS can distinguish between a protein located within the gap junction and a protein bound to the nanoparticle outside the gap (see schematics enclosed in blue and green rectangles in figure below, respectively); these results are compared with the SERS signal from aggregated gold nanoparticles (schematic enclosed in red rectangle below).  Furthermore, they saw an effect of nanoparticle probe size on absolute signal indicating either larger enhancements with larger particles or increased number of sample molecules.  The ability to enhance Raman scattering from molecules outside the gap junction by using functionalized nanoparticles provides a way to increase sensitivity in TERS experiments from complicated biological samples by providing another platform for preparing complex biological molecules that can be probed by the ultrasensitive TERS method.

Thursday, 25 July 2013 09:07

Excellence in Nanoindentation

 Standard AFM technology is currently deficient when it comes to  nanoindentation. During indentation, a sample that is in contact with a tip  moves up against the tip for the indentation operation. The sample indents  a given distance as  a result of the force, butthe critical force is unknown,  since standard cantilever structures bend. Thus, the distance indented  includes both the sample indentation depth and the cantilever bending.  Nanonics has solved this problem with its Multiprobe AFM Systems. MultiProbes allow straight diamond or other indenters and frees AFM nanoindentation from cantilever bending constraints.

Straight indenters of any type can be bonded to tuning forks, allowing them to be brought in contact with the working surface, while a second AFM probe monitors the indenter’s Z-axis alteration at and only at the straight indenter as the sample is lifted in the Z direction against the indenter. As a result, only the Z-axis alteration of the straight indenter is monitored, independent of any possible tuning fork bending that may occur in spite of the >5000 N/m Force Constant. Up to four probes can be added for on-line monitoring of electrical and thermal parameters during indentation.


For the first time, a state of the art AFM measures true indentation depth versus load, allowing the user to learn:

  • Young’s modulus
  • Indentation Modulus
  • Indentation Creep
  • Indentation Creep
  • Indentation Hardness
  • Progressive Load
  • Constant Load
  • Multipass Scratch & Wear Tests with Variable Force
  • Including Force-displacement curves and scratch and
    wear tests with variable force, rate, length  and direction
  • Rate, Length and Direction
  • Environmental & temperature control
  • High force constant (100 to300 N/m) quartz conical
    indenters with customization
  • Nanoheater nanoindenters
  • NanoIndenters with on-line Raman spectroscopy of
     vibrational alterations


Different Indentation Protocols

Array of Nanoindentation with various controlled force


Nano-Indentation Software module for various NanoIndentation Protocols and with flexible interface for user scripting




On-line Load and Unload Curves Versus Distance



The Choice Is Yours From Various Indentation To Scratch Protocols

Scanning Probe Microscopy NanoIndentaion array. Bar is 1.7 µm

A close-up AFM image of  NanoIndentaion array. Bar is 750 nm

AFM image if a nano-scratch performed with Nanonics MultiView Nanoindentation system. bar is 3.0 µm



Variety Of Probes For Hard & Soft Indentation:  Shown Indentation of Gold A Soft Material With Nanonics Unique Connical Glass Probes

3D representation of the topographic images before and after Nanoindentation process.  Indentation (A) obtained with indenting connical 30 nm glass probe  and imaging (B) done on-line with second super-resolution high aspect ratio probe



Ultrahigh Control Of Load Parameters and Indentation Depth

NanoIndentation obtained on SiO2 thin layer in Si obtained with 50nm Berkovich Diamond tip:
A) AFM topographic image of a nanoindentation obtained with a load of100µN.

B) Line profile crossing the nanoindentation areas shows a depth of 36.5nm

C) AFM topographic image of a nanoindentation obtained with a load of50µN.

D) Line profile crossing the nanoindentation areas shows a depth of 15.08nm


On-line Raman Chemical Analysis

On-line AFM/Raman Characteristics of Silicon Indentation

a) AFM image of  Silicon Indentation

b) Indentation Height line profile

c) On-line Raman Spectra at marked pints on b showing the different stress level of the indentation patterns

Wednesday, 24 July 2013 13:11

Tip-Enhanced Raman Scattering of Probe

Tip-enhanced Raman scattering of  70 nm strained silicon layer single gold nanoparticle probe


Line profile Raman intensity of sSi silicon mesa:

Blue: Far-field

Green: Near-field

Red: Difference



Wednesday, 24 July 2013 13:06

Single Bio-Molecules TERS

Wednesday, 24 July 2013 13:02

Investigating Reversible Defect in Graphene

Reversible Defect in Graphene Investigated by Tip-Enhanced Raman Spectroscopy

Wednesday, 24 July 2013 12:55

AFM/Raman in Liquid Cell

  • Clear Optical Axis from Above
  • Tuning Fork feedback with no optical interference and with low working distance suitable for Water Immersion Objective integration
  • Hight Magnification and Large NA objective for online AFM/NSOM/Raman with high optical throughput.
  • Flexible liquid cell geometry and size. Suitable for petri dish.


Wednesday, 24 July 2013 12:53

AFM/Raman Imaging of Graphene Layers

AFM/Raman Imaging of Graphene

Single and Double  Layers

AFM/Raman of Carbon Nanotube

Chemical Mapping Using Raman Microscopy

AFM Raman of CNT Nanowire on Silicon

The frequency of the Raman band at 1575cm-1 indicates whether

the carbon nanotube is at a metallic, semi-conductor, or insulating

orientation. Clear difference is observed between the high quality

nanotube (red) and the disordered material (green).

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